InSplorer Endoscope - Lightcore Technologies

Flexible Probe for Multiphoton Multimodal Imaging

InSplorer endoscope enables multimodal nonlinear imaging such as 2-photon and 3-photon fluorescence but also harmonic generation (SHG, THG) and coherent Raman imaging (CARS).

CARS/SHG – Humain Colon Lipid(Red) and Collagen(Green)

Mouse spinal cord: Myelin (magenta – CARS), spinal axon (blue – 2photon)

Human skin: stratum corneum (autofluorescence)

Mouse brain labelled with GCamp (2photon)

Epidermal layer of unstained mouse skin. Collagen fibrils structure (SHG – green) ; Cellular structure (3PEF – red, THG – blue)

GFP labeled mouse brain (3PEF – λexc = 1300 nm)

Mouse retina inner plexiform layer (3PEF – λexc = 1300 nm)

InSplorer probe is connected to a standalone cabinet that houses the laser, opto-electronics and computer. The InSplorer system is powered by a dedicated, user-friendly software.

InSplorer multiphoton endoscope can also use your favorite table-top multiphoton laser using the Lightcore FemtoGuide Fiber

Main Specifications

Available laser sources:

Pulsed femtosecond and picosecond lasers
Wavelength range: 750 nm to 1550 nm
Integration of your existing light source using our FemtoGuide Fiber

Imaging performances:

Frame rate: up to 10 Hz
Field of view: up to 600 µm
PSF: 1 µm
Collection NA: up to 0.7
Working distance: up to 600 µm
Imaging modalities (Fluorescence and label free): 2-Photon, 3-photon, SHG, THG, CARS

Microendoscope specifications:

Tip diameter: 1.3 mm or 2.2 mm (distal)
Distal end length: <40 mm
Flexible part length: 50-400 cm
Weight (distal end): < 2gr

brochure-InSplorer-2022-FINAL Download

Publications

D. Septier, V. Mytskaniuk, R. Habert, D. Labat, K. Baudelle, A. Cassez, G. Brévalle-Wasilewski, M. Conforti, G. Bouwmans, H. Rigneault, and A. Kudlinski, “Label-free highly multimodal nonlinear endoscope,” Optics Express 30, 25020-25033 (2022). https://doi.org/10.1364/OE.462361

A. Kudlinski, A. Cassez, O. Vanvincq, D. Septier, A. Pastre, R. Habert, K. Baudelle, M. Douay, V. Mytskaniuk, V. Tsvirkun, H. Rigneault, and G. Bouwmans, “Double clad tubular anti-resonant hollow core fiber for nonlinear microendoscopy,” Optics Express 28, 15062-15070 (2020). https://doi.org/10.1364/OE.389084

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Science & Applications 7, 10 (2018). https://doi.org/10.1038/s41377-018-0003-3

A. Lombardini, E. R. Andresen, A. Kudlinski, I. Rimke, and H. Rigneault, “Origin and suppression of parasitic signals in Kagomé lattice hollow core fibers used for SRS microscopy and endoscopy,” Optics Letters 42, 1824-1827 (2017). https://doi.org/10.1364/OL.42.001824